Hygromycin B Powder Size:5g 3 g/L Store at room

3  g/L Store at room temperature

Chemical formula C5H5N5-O

0 (10 g/l) Assay ≥99%

01 Suspend 22 gram powdered medium in 1 litre distilled water

Dilute 20ml TAE Buffer 50X with 980ml deionised water to make 1 litre of TAE Buffer

Hygromycin B Powder Size:5g 3 g/L Store at roomEmpirical Formula (Hill Notation): C20H37N3O13 Molecular Weight = 527. 5 Hygromycin B is an aminoglycoside produced by Streptomyces hygroscopicus. The antibiotic interferes with the translation step of polypeptide synthesis of prokaryotes and eukaryotes. The antibiotic inhibits peptide chain elongation by preventing elongation factor EF 2 dependent translocation. Hygromycin B is used as a selective agent in yeasts, bacterial and plant cells. The