Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Thiamine Size:500g Molecular cloning: a laboratory manual

Molecular cloning: a laboratory manual

2 at 25°C Suspend 60 gram powdered medium in 1 litre distilled water

Pre-Sporulation Medium is used for strains that do not sporulate well when grown on sporulation medium directly

This might cause problems in osmo-molarity and intercellular Potassium ion concentrations finally resulting in poor cell growth and low yields

2 Glucose 2 KH2PO4 1

Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Thiamine Size:500g Molecular cloning: a laboratory manualThiamine HCl is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of thiamine or to replace this vitamin. Ammonium sulphate, as a source of Nitrogen, has been omitted to test the ability of yeast cells to assimilate different nitrogen sources like Potassium nitrate. Suspend 1. 9 gram powdered medium in 1 litre distilled water Store dry at room