SD Broth / 2% Glucose w/o Phosphate (KH2PO4) Size:500g The concentration of NaCl in

The concentration of NaCl in L-Broth is low compared to both LB Miller and LB Lennox formulations

trace elements and salts resulting in a good development of yeast colonies

Tryptone 10 Yeast extract 5 NaCl 0

at this strength the ph is around 6

32 KH2PO4 870 Carbon Source   Glucose 10000 Total 19334

SD Broth / 2% Glucose w/o Phosphate (KH2PO4) Size:500g The concentration of NaCl inSD Broth 2% Glucose w o Phosphate (KH2PO4) is based upon Yeast Nitrogen base without Amino acids and including glucose. The absence of phosphate (KH2PO4) provides the possibility to include alternative sources of phosphate, like P32 labeled components, or to select an optimal concentration of phosphate for certain yeast cell lines. Suspend 25. 9 gram powdered medium in 1 litre distilled water Store dry at room temperature